Development of a rGO-doped polyaniline biosensor to detect glucose
DOI:
https://doi.org/10.71701/b741pz21Keywords:
Enzymatic biosensor, polyaniline PAni, reduced graphene oxide rGO, glucose oxidase, redox reaction, cyclic voltamperometryAbstract
In this work, electrochemical sensors were designed and manufactured using a Printed Circuit Board coated with copper foil as substrate. These sensors were developed using a matrix of the conductive polymer polyaniline and the nanostructured compound of reduced graphene oxide, and were applied two different drying methods: infrared light and spin coating. Then, the glucose oxidase enzyme of high specificity was immobilized with glutaraldehyde solution. The sensors were analyzed in the presence of a D-Glucose solution at a concentration of 16 mM. The electrochemical activity was measured using the cyclic voltamperometry technique. As for the results, those samples dried with infrared light displayed better performance at scan rates of 0.3 to 0.8 mV/s with a high charge transfer of 0.8 mV/s and reversibility. On the other hand, the electrodes dried with the spin coating method did not show reversibility. With this technique it is highly likely to develop biosensors to detect glucose in diabetic people in Peru.
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